Identification and determination of the two principal metabolites of minocycline in humans
Summary
Type: HPLC Application #: 103780
Catalog#: 720023.46 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4.6x250mm
Detection: UV, 352nm Conditions: eluent: gradient from 10 to 40% acetonitrile in 0.2% formic acid within 5min, then 8μin 40% acetonitrile flow rate 1.2ml/min Authors: Böcker R H Source: J Chromatogr 568 (1991) 363 - 374
Details
Legend: Fig. 1. HPLC determination of minocycline in human serum and urine, A) blank
serum spiked with 1µg/ml minocycline, B) serum at 0.5h, C) serum at 1.5h, D)
serum at 24h, E) urine at 0 - 12h, F) urine